GlycoSens™ Citrate Synthase (CS) Assay Kit, Micro-method

The GlycoSens™ Citrate Synthase (CS) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target citrate synthase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Glyoxylic Acid Cycle Series

CS

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

50 μL

The assay relies on the distinct spectroscopic optical characteristics of citrate synthase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect CS content in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Citrate Synthase

Citrate Synthase; CS

Functioning at the core of various biological processes, citrate synthase (CS) is a key subject of biochemical analysis. Its enzymatic function is indispensable for the efficient generation of cellular biomass and storage reserves. Thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Designed for comprehensive functional analysis of citrate synthase (CS) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Enhanced Sensitivity; Streamlined Workflow; Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.