GlycoSens™ Citrate Synthase (CS) Assay Kit, Microplate Method

The GlycoSens™ Citrate Synthase (CS) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target citrate synthase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

CS

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of citrate synthase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect CS content in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Citrate Synthase

Citrate Synthase; CS; Citrate Synthase Standard; Citrate Synthase Reagent

Functioning at the core of various biological processes, citrate synthase (CS) is a key subject of biochemical analysis. By catalyzing the covalent linkage of its substrates, it supports intracellular synthesis requirements. Notably, assessing this component offers a reliable parameter for agricultural research or biological studies.

Specifically engineered for evaluating citrate synthase (CS) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

High Specificity; Rapid Reaction Protocol; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.