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GlycoSens™ Creatine Assay Kit, Micro-method

CAT#: GLJF-0926-HX1468

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Specifications
Product Description
The GlycoSens™ Creatine Assay Kit utilizes a highly stable detection system tailored for accurate profiling of creatine. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.
Product Type
Other Series
Target
Creatine
Kit Components
Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution
Sample Types
Tissues, other biological samples
Sample Volume
10 μL - 50 μL
Detection Principle
Target creatine in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target creatine.
Detection Time
30-60 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Creatine
Alternate Names
Creatine
Application Notes
Background
In the context of biological research, creatine has emerged as a molecule of high interest. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. Consequently, accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.
Application
Essential for monitoring creatine level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.
Key Features & Advantages
Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.
For Research Use Only.
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