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The GlycoSens™ Creatine Assay Kit offers an advanced quantitative methodology for evaluating creatine concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Other Series
Target
Creatine
Kit Components
Sample Lysis Buffer; Substrate; Detection Reagent; Standard
Sample Types
Tissues, other biological samples
Sample Volume
5 μL - 20 μL
Detection Principle
The system measures chromatic product formation derived from target creatine activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of creatine levels.
Detection Time
Within 45 minutes
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Creatine
Alternate Names
Creatine
Application Notes
Background
Known for its specific biological characteristics, creatine provides deep insights into cellular dynamics. It forms a crucial part of the cellular inventory, dynamically responding to internal and external stimuli. Furthermore, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.
Application
Designed for comprehensive functional analysis of creatine within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.