GlycoSens™ Creatine Assay Kit, Enzymatic Method

The GlycoSens™ Creatine Assay Kit utilizes a highly stable detection system tailored for accurate profiling of creatine. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Basal Metabolic Series

Creatine

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

Target creatine in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target creatine.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Creatine

Creatine

Creatine is an integral element required for robust cellular function and metabolism. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. As a result, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Designed for comprehensive functional analysis of creatine within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

High Specificity; Stable Reaction; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.