GlycoSens™ Hydroxyproline (Hyp) Assay Kit (for serum and tissue)

The GlycoSens™ Hydroxyproline (Hyp) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of hydroxyproline levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

Hyp

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, serum

10 μL - 50 μL

Quantification of hydroxyproline (Hyp) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydroxyproline

Hydroxyproline; Hyp; Hydroxyproline Standard

The presence and activity of hydroxyproline (Hyp) are closely linked to overall organismal health. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Specifically engineered for evaluating hydroxyproline (Hyp) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Optimized Reaction Dynamics; Excellent Reproducibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.