GlycoSens™ Hydroxyproline (Hyp) Assay Kit (for culture medium)

The GlycoSens™ Hydroxyproline (Hyp) Assay Kit offers an advanced quantitative methodology for evaluating hydroxyproline concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Oxidation and Antioxidant Series

Hyp

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The system measures chromatic product formation derived from target hydroxyproline activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of Hyp levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydroxyproline

Hydroxyproline; Hyp; Hydroxyproline Standard; Hydroxyproline Reagent

Found widely across biological samples, hydroxyproline (Hyp) is crucial for maintaining systemic homeostasis. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Tailored for accurate quantification of hydroxyproline (Hyp) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Stable Signal Output; Broad Sample Compatibility; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.