GlycoSens™ Hydroxyproline (Hyp) Assay Kit

The GlycoSens™ Hydroxyproline (Hyp) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target hydroxyproline in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

Hyp

Assay Buffer; Enzyme Mix; Developer Solution; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of hydroxyproline. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect Hyp content in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydroxyproline

Hydroxyproline; Hyp; Hydroxyproline Standard; Hydroxyproline Reagent

Hydroxyproline (Hyp) is an integral element required for robust cellular function and metabolism. This constituent influences multiple downstream effects, anchoring the stability of interrelated metabolic chains. In addition, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Optimized for high-throughput profiling of hydroxyproline (Hyp) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Optimized Reaction Dynamics; Excellent Reproducibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.