GlycoSens™ Hydroxyproline (HYP) Assay Kit, Micro-method

The GlycoSens™ Hydroxyproline (HYP) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of hydroxyproline (HYP) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Amino Acid Metabolism Series

HYP

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

5 μL - 20 μL

Quantification of hydroxyproline (HYP) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Hydroxyproline

Hydroxyproline; HYP

Found widely across biological samples, hydroxyproline (HYP) is crucial for maintaining systemic homeostasis. This constituent influences multiple downstream effects, anchoring the stability of interrelated metabolic chains. Consequently, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Ideal for investigating hydroxyproline (HYP) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.