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The GlycoSens™ Lactic Acid (LD) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of lactic acid (LD) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Quantification of lactic acid (LD) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Detection Wavelength
530 nm
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Found widely across biological samples, lactic acid (LD) is crucial for maintaining systemic homeostasis. This acid derivative plays a structural and functional role in maintaining cellular pH and metabolic flow. In addition, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Optimized for high-throughput profiling of lactic acid (LD) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.