GlycoSens™ N-Acetyl-β-D-glucosidase (NAG) Assay Kit, Micro-method

The GlycoSens™ N-Acetyl-β-D-glucosidase (NAG) Assay Kit offers an advanced quantitative methodology for evaluating N-acetyl-β-d-glucosidase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Carbohydrate Metabolism Series

NAG

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

20 μL - 100 μL

The system measures chromatic product formation derived from target N-acetyl-β-d-glucosidase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of NAG levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

N-Acetyl-β-D-glucosidase

N-Acetyl-β-D-glucosidase; NAG

N-Acetyl-β-D-glucosidase (NAG) acts as an essential biochemical entity within diverse cellular pathways. It effectively accelerates specific biochemical transformations, ensuring the rapid progression of metabolic pathways. Consequently, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Designed for comprehensive functional analysis of N-acetyl-β-d-glucosidase (NAG) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.