GlycoSens™ N-Acetyl-β-D-glucosidase (NAG) Assay Kit, Spectrophotometry

The GlycoSens™ N-Acetyl-β-D-glucosidase (NAG) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target N-acetyl-β-d-glucosidase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Carbohydrate Metabolism Series

NAG

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

20 μL - 100 μL

The assay relies on the distinct spectroscopic optical characteristics of N-acetyl-β-d-glucosidase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect NAG content in the sample.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

N-Acetyl-β-D-glucosidase

N-Acetyl-β-D-glucosidase; NAG

As a pivotal biomolecule, N-acetyl-β-D-glucosidase (NAG) plays a central role in complex physiological networks. It selectively processes distinct biochemical substrates, maintaining the efficiency of cellular networks. Furthermore, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Essential for monitoring N-acetyl-β-d-glucosidase (NAG) level shifts during molecular biochemistry, metabolic pathway flux analysis, and enzymology studies.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.