GlycoSens™ Proline Dehydrogenase (ProDH) Assay Kit, Micro-method

The GlycoSens™ Proline Dehydrogenase (ProDH) Assay Kit offers an advanced quantitative methodology for evaluating proline dehydrogenase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Amino Acid Metabolism Series

ProDH

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

20 μL - 100 μL

The system measures chromatic product formation derived from target proline dehydrogenase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of ProDH levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Proline Dehydrogenase

Proline Dehydrogenase; ProDH; Proline Oxidoreductase

The presence and activity of proline dehydrogenase (ProDH) are closely linked to overall organismal health. Its enzymatic action supports metabolic redox homeostasis and efficient energy conversion pathways. Consequently, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Optimized for high-throughput profiling of proline dehydrogenase (ProDH) in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

Excellent Reproducibility; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.