GlycoSens™ Proline Dehydrogenase (ProDH) Assay Kit, Spectrophotometry

The GlycoSens™ Proline Dehydrogenase (ProDH) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of proline dehydrogenase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Amino Acid Metabolism Series

ProDH

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

Target proline dehydrogenase (ProDH) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target ProDH.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Proline Dehydrogenase

Proline Dehydrogenase; ProDH; Proline Oxidoreductase

Proline dehydrogenase (ProDH) acts as an essential biochemical entity within diverse cellular pathways. It catalyzes critical oxidation-reduction reactions by transferring hydride groups to specific electron acceptors. Tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Widely applicable for tracking proline dehydrogenase (ProDH) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.