GlycoSens™ Pullulanase Assay Kit

The GlycoSens™ Pullulanase Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target pullulanase in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Carbohydrate Metabolism Series

Pullulanase

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

50 μL

The assay relies on the distinct spectroscopic optical characteristics of pullulanase. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect pullulanase content in the sample.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pullulanase

Pullulanase

Pullulanase is an integral element required for robust cellular function and metabolism. Its enzymatic activity is finely tuned to environmental cues, reflecting the overall metabolic capacity. Accurate quantification of this target is essential for uncovering underlying pathological and physiological mechanisms.

Tailored for accurate quantification of pullulanase across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Superior Accuracy; Excellent Reproducibility; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.