GlycoSens™ Pyruvate Dehydrogenase (PDH) Assay Kit, Micro-method

The GlycoSens™ Pyruvate Dehydrogenase (PDH) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of pyruvate dehydrogenase (PDH) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Tricarboxylic Acid Cycle Series

PDH

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

5 μL - 20 μL

Quantification of pyruvate dehydrogenase (PDH) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pyruvate Dehydrogenase

Pyruvate Dehydrogenase; PDH; Pyruvate Oxidoreductase

Research highlights pyruvate dehydrogenase (PDH) as a significant participant in key metabolic and regulatory cascades. By mediating the transfer of electrons, it strictly regulates intermediate metabolic fluxes. Therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Designed for comprehensive functional analysis of pyruvate dehydrogenase (PDH) within tissue metabolism profiling, coenzyme utilization studies, and biochemical pathway modeling.

Robust Assay Design; Easy-to-Follow Protocol; Versatile Application Range

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.