GlycoSens™ Pyruvate Dehydrogenase (PDH) Assay Kit

The GlycoSens™ Pyruvate Dehydrogenase (PDH) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of pyruvate dehydrogenase (PDH) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Tricarboxylic Acid Cycle Series

PDH

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

Quantification of pyruvate dehydrogenase (PDH) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pyruvate Dehydrogenase

Pyruvate Dehydrogenase; PDH; Pyruvate Oxidoreductase

Functioning at the core of various biological processes, pyruvate dehydrogenase (PDH) is a key subject of biochemical analysis. It catalyzes critical oxidation-reduction reactions by transferring hydride groups to specific electron acceptors. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Tailored for accurate quantification of pyruvate dehydrogenase (PDH) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Linear Dynamic Range; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.