GlycoSens™ Pyruvate Dehydrogenase (PDH) Assay Kit, Spectrophotometry

The GlycoSens™ Pyruvate Dehydrogenase (PDH) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of pyruvate dehydrogenase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Tricarboxylic Acid Cycle Series

PDH

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

Target pyruvate dehydrogenase (PDH) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target PDH.

30-60 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Pyruvate Dehydrogenase

Pyruvate Dehydrogenase; PDH; Pyruvate Oxidoreductase

The presence and activity of pyruvate dehydrogenase (PDH) are closely linked to overall organismal health. It is responsible for driving the oxidative conversion of its substrate, typically utilizing NAD or FAD as cofactors. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Ideal for investigating pyruvate dehydrogenase (PDH) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.