GlycoSens™ Sucrose Phosphate Synthase (SPS) Assay Kit, Spectrophotometry

The GlycoSens™ Sucrose Phosphate Synthase (SPS) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of sucrose phosphate synthase (SPS) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Sucrose Series

SPS

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Quantification of sucrose phosphate synthase (SPS) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Sucrose Phosphate Synthase

Sucrose Phosphate Synthase; SPS

Known for its specific biological characteristics, sucrose phosphate synthase (SPS) provides deep insights into cellular dynamics. By catalyzing the covalent linkage of its substrates, it supports intracellular synthesis requirements. Tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Specifically engineered for evaluating sucrose phosphate synthase (SPS) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

High Specificity; Micro-Volume Optimization; Broad Sample Compatibility

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.