GlycoSens™ Total Antioxidant Capacity (ABTS Method) Assay Kit, Spectrophotometry

The GlycoSens™ Total Antioxidant Capacity Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of total antioxidant capacity levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Oxidation and Antioxidant Series

Substrate Mix A; Substrate Mix B; Standard; Reaction Stop Solution

Serum, plasma, tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

Quantification of total antioxidant capacity (ABTS method) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 1 hour

734 nm

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Total antioxidant capacity is an integral element required for robust cellular function and metabolism. This collective metric is highly reflective of broad metabolic shifts and systemic physiological adaptations. Notably, tracking its dynamics facilitates a deeper understanding of cellular responses to external stressors.

Widely applicable for tracking total antioxidant capacity fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

High Specificity; Broad Sample Compatibility; Stringent Quality Control

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.