GlycoSens™ Isoleucine Assay Kit

The GlycoSens™ Isoleucine Assay Kit utilizes a highly stable detection system tailored for accurate profiling of isoleucine. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Amino Acid Metabolism Series

Isoleucine

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

20 μL - 100 μL

Target isoleucine in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target isoleucine.

Within 1 hour

High-performance liquid chromatograph, centrifuge, chromatographic column, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Isoleucine

Isoleucine

Research highlights isoleucine as a significant participant in key metabolic and regulatory cascades. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Furthermore, detailed analysis of this target empowers researchers to monitor disease progression or developmental stages effectively.

Optimized for high-throughput profiling of isoleucine in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.

High Sensitivity & Specificity; Flexible Sample Inputs; Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.