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The GlycoSens™ Trehalose (TREH) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target trehalose in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Other Series
Target
TREH
Kit Components
Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard
Sample Types
Tissues, other biological samples
Sample Volume
50 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of trehalose. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect TREH content in the sample.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Trehalose
Alternate Names
Trehalose; TREH
Application Notes
Background
Found widely across biological samples, trehalose (TREH) is crucial for maintaining systemic homeostasis. It regulates osmotic balance and provides readily available energy reserves for cellular functions. Consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.
Application
Widely applicable for tracking trehalose (TREH) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.
Key Features & Advantages
High Specificity; Micro-Volume Optimization; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.