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The quantitative anti-β2 glycoprotein 1 screen ELISA kit measures the concentration of unknown auto-antibodies against the β2-glycoprotein 1 complex using two different calibration curves and peroxidase conjugates (for IgG and IgM, respectively). Antibodies in the sample bind to β2-glycoprotein 1 coated on microplate wells, followed by incubation and washing to remove non-reactive components. The color reaction produced by the enzymatic reaction quantifies the concentration of antibodies in the sample.
The quantitative anti-β2 glycoprotein 1 screen ELISA kit is applied in clinical research and is often used to detect and measure the presence of anti-β2-glycoprotein 1 antibodies, which are often associated with autoimmune diseases such as antiphospholipid syndrome (APS).