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Quantitative Human C5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX132

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Specifications
Product Description
The quantitative human C5 (glycosylated) sandwich ELISA kit is designed to detect human complement component 5 (C5) levels. C5 is a crucial protein in the complement system, playing a central role in the terminal pathway leading to membrane attack complex formation and inflammation. It's cleavage into C5a and C5b initiates powerful immune responses. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 1.623 ng/mL, which can accurately detect low concentrations of C5 in the sample.
Target
C5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
1.623 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
5 ng/mL-200 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Complement component 5
Alternate Names
C5; Complement component 5; CPAMD4; C5a anaphylatoxin
Uniprot No.
N-Glycosylation Site
741, 911, 1115, 1630
Application Notes
Application
The quantitative human C5 (glycosylated) sandwich ELISA kit is essential for research investigating the role of C5 in immune responses, inflammatory diseases, and autoimmune disorders. Researchers use this ELISA to quantify C5 levels, providing valuable insights into its involvement in various pathological processes and potential therapeutic targets.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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