Quantitative Human C5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX132)

Product Description	The quantitative human C5 (glycosylated) sandwich ELISA kit is designed to detect human complement component 5 (C5) levels. C5 is a crucial protein in the complement system, playing a central role in the terminal pathway leading to membrane attack complex formation and inflammation. It's cleavage into C5a and C5b initiates powerful immune responses. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 1.623 ng/mL, which can accurately detect low concentrations of C5 in the sample.
Target	C5
N-Glycosylation Site	741, 911, 1115, 1630
Sample Types	Serum, plasma
Sample Volume	100 μL
Sensitivity	1.623 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	5 ng/mL-200 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Complement component 5
Alternate Names	C5; Complement component 5; CPAMD4; C5a anaphylatoxin
Uniprot No.	P01031

Application	The quantitative human C5 (glycosylated) sandwich ELISA kit is essential for research investigating the role of C5 in immune responses, inflammatory diseases, and autoimmune disorders. Researchers use this ELISA to quantify C5 levels, providing valuable insights into its involvement in various pathological processes and potential therapeutic targets.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 90-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.