Quantitative Human CTSB (Glycosylated) Sandwich ELISA Kit

The quantitative human CTSB (glycosylated) sandwich ELISA kit is designed to detect human cathepsin B (CTSB) levels. CTSB is a lysosomal cysteine protease that plays a role in intracellular protein degradation and processing, participating in various cellular functions, including antigen presentation and bone remodeling. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.153 ng/mL, which can accurately detect low concentrations of CTSB in the sample.

CTSB

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.153 ng/mL

Quantitative sandwich ELISA

0.55 ng/mL-20 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Cathepsin B

CTSB; Cathepsin B; APPS; CPSB

192

The quantitative human CTSB (glycosylated) sandwich ELISA kit is valuable in research investigating cancer metastasis, arthritis, and other diseases where CTSB is implicated in tissue remodeling and degradation. Researchers utilize this ELISA to quantify CTSB levels, providing insights into its role in disease pathogenesis and potential therapeutic targeting.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 95-110%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.