Quantitative Human CTSB (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX192)

Product Description	The quantitative human CTSB (glycosylated) sandwich ELISA kit is designed to detect human cathepsin B (CTSB) levels. CTSB is a lysosomal cysteine protease that plays a role in intracellular protein degradation and processing, participating in various cellular functions, including antigen presentation and bone remodeling. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.153 ng/mL, which can accurately detect low concentrations of CTSB in the sample.
Target	CTSB
N-Glycosylation Site	192
Sample Types	Tissue homogenates, serum, plasma
Sample Volume	100 μL
Sensitivity	0.153 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	0.55 ng/mL-20 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Cathepsin B
Alternate Names	CTSB; Cathepsin B; APPS; CPSB
Uniprot No.	P07858

Application	The quantitative human CTSB (glycosylated) sandwich ELISA kit is valuable in research investigating cancer metastasis, arthritis, and other diseases where CTSB is implicated in tissue remodeling and degradation. Researchers utilize this ELISA to quantify CTSB levels, providing insights into its role in disease pathogenesis and potential therapeutic targeting.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 95-110%; Plasma sample: n=4, 85-100%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.