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Quantitative Human GDF9 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX70

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Specifications
Product Description
The quantitative human GDF9 (glycosylated) sandwich ELISA kit is designed to detect human growth differentiation factor 9 (GDF9) levels. GDF9 is a secreted protein belonging to the TGF-β superfamily, primarily known for its essential role in ovarian folliculogenesis and oocyte maturation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.601 ng/mL, which can accurately detect low concentrations of GDF9 in the sample.
Target
GDF9
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.601 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Growth differentiation factor 9
Alternate Names
GDF9; Growth differentiation factor 9; GDF-9
Uniprot No.
N-Glycosylation Site
106, 163, 236, 255, 268, 338
Application Notes
Application
The quantitative human GDF9 (glycosylated) sandwich ELISA kit is designed to measure the concentration of glycosylated GDF9 in biological samples. This application is crucial for research investigating female fertility, ovarian function, and related reproductive disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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