Quantitative Human JAG1 (Glycosylated) Sandwich ELISA Kit

The quantitative human JAG1 (glycosylated) sandwich ELISA kit is designed to detect human jagged 1 protein (JAG1) levels. JAG1 is a transmembrane protein that plays a vital role in the Notch signaling pathway. As a ligand, JAG1 interacts with Notch receptors on neighboring cells, initiating a cascade of intracellular events. This signaling process is fundamental for numerous developmental processes, including cell fate determination, tissue patterning, and stem cell maintenance. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.068 ng/mL, which can accurately detect low concentrations of JAG1 in the sample.

JAG1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.068 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Jagged 1 protein

JAG1; Jagged 1 protein; CD339; AGS; JAGL1; Alagille syndrome

143, 217, 382, 559, 745, 960, 991, 1045, 1064

The quantitative human JAG1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated JAG1 levels in various biological samples. Researchers can employ this kit to investigate the involvement of JAG1 in various physiological and pathological conditions. It proves useful in studies exploring Notch signaling in development, cancer, and other diseases. It has applications in developmental biology research, oncology, and studies related to cell signaling pathways.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.