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Quantitative Human METAP2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX445)

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1×48 T
1×96 T

Product Description The quantitative human METAP2 (glycosylated) sandwich ELISA kit is designed to detect human methionyl aminopeptidase 2 (METAP2) levels. METAP2 is an enzyme that removes the N-terminal methionine residue from newly synthesized proteins. This processing step is important for protein functions. METAP2 is involved in regulating protein synthesis. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of METAP2 in the sample.
Target METAP2
O-Glycosylation Site 60, 63
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.085 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Methionyl aminopeptidase 2
Alternate Names METAP2; Methionyl aminopeptidase 2; MNPEP; Peptidase M
Uniprot No. P50579
Application The quantitative human METAP2 (glycosylated) sandwich ELISA kit is used to measure glycosylated METAP2 levels in samples. This measurement is important in research related to protein metabolism. The kit is applied in studies of cellular processes, which helps in understanding the role of METAP2.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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