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Quantitative Human METAP2 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX445

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Specifications
Product Description
The quantitative human METAP2 (glycosylated) sandwich ELISA kit is designed to detect human methionyl aminopeptidase 2 (METAP2) levels. METAP2 is an enzyme that removes the N-terminal methionine residue from newly synthesized proteins. This processing step is important for protein functions. METAP2 is involved in regulating protein synthesis. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of METAP2 in the sample.
Target
METAP2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.085 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Methionyl aminopeptidase 2
Alternate Names
METAP2; Methionyl aminopeptidase 2; MNPEP; Peptidase M
Uniprot No.
O-Glycosylation Site
60, 63
Application Notes
Application
The quantitative human METAP2 (glycosylated) sandwich ELISA kit is used to measure glycosylated METAP2 levels in samples. This measurement is important in research related to protein metabolism. The kit is applied in studies of cellular processes, which helps in understanding the role of METAP2.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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