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Quantitative Human MRF (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX912

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Specifications
Product Description
The quantitative human MRF (glycosylated) sandwich ELISA kit is designed to detect human myelin gene regulatory factor (MRF) levels. MRF is a transcription factor. MRF plays a crucial role in the development and maintenance of myelin, the insulating sheath that surrounds nerve fibers. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.085 ng/mL, which can accurately detect low concentrations of MRF in the sample.
Target
MRF
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.085 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Myelin gene regulatory factor
Alternate Names
MRF; Myelin gene regulatory factor; C11orf9; Chromosome 11 open reading frame 9; MRF_HUMAN
Uniprot No.
N-Glycosylation Site
919, 1043, 1065, 1129
Application Notes
Application
The quantitative human MRF (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated MRF levels in biological samples. The assay is valuable for investigating the role of MRF in myelination, neurological disorders, and nerve development.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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