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Quantitative Human NHE1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX277)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NHE1 (glycosylated) sandwich ELISA kit is designed to detect human sodium-hydrogen exchanger 1 (NHE1) levels. NHE1 is a protein that is found in the plasma membrane of cells. This protein plays a role in regulating intracellular pH. It is involved in cell volume regulation and ion transport. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.075 ng/mL, which can accurately detect low concentrations of NHE1 in the sample.
Target NHE1
N-Glycosylation Site 75
O-Glycosylation Site 42, 56, 61, 62, 68
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.075 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sodium-hydrogen exchanger 1
Alternate Names NHE1; Sodium-hydrogen exchanger 1
Uniprot No. P19634
Application The quantitative human NHE1 (glycosylated) sandwich ELISA kit provides a means for the quantitative analysis of glycosylated NHE1 levels in biological samples. Researchers use it in studies investigating hypertension, heart failure, and cancer. The kit is applicable in cardiovascular research.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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