Quantitative Human NHE1 (Glycosylated) Sandwich ELISA Kit

The quantitative human NHE1 (glycosylated) sandwich ELISA kit is designed to detect human sodium-hydrogen exchanger 1 (NHE1) levels. NHE1 is a protein that is found in the plasma membrane of cells. This protein plays a role in regulating intracellular pH. It is involved in cell volume regulation and ion transport. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.075 ng/mL, which can accurately detect low concentrations of NHE1 in the sample.

NHE1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.075 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Sodium-hydrogen exchanger 1

NHE1; Sodium-hydrogen exchanger 1

75

42, 56, 61, 62, 68

The quantitative human NHE1 (glycosylated) sandwich ELISA kit provides a means for the quantitative analysis of glycosylated NHE1 levels in biological samples. Researchers use it in studies investigating hypertension, heart failure, and cancer. The kit is applicable in cardiovascular research.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.