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Quantitative Human NOX1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX951

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Specifications
Product Description
The quantitative human NOX1 (glycosylated) sandwich ELISA kit is designed to detect human nicotinamide adenine dinucleotide phosphate oxidase 1 (NOX1) levels. NOX1 is an enzyme that generates reactive oxygen species (ROS). ROS are involved in various cellular processes. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.097 ng/mL, which can accurately detect low concentrations of NOX1 in the sample.
Target
NOX1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.097 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Nicotinamide adenine dinucleotide phosphate oxidase 1
Alternate Names
NOX1; Nicotinamide adenine dinucleotide phosphate oxidase 1; NOH1; NADPH oxidase 1
Uniprot No.
N-Glycosylation Site
162, 236
Application Notes
Application
The quantitative human NOX1 (glycosylated) sandwich ELISA kit is used to measure NOX1 levels in biological samples. The assay enables the study of NOX1's role in oxidative stress, cell signaling, and vascular biology.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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