Quantitative Human OGT (Glycosylated) Sandwich ELISA Kit

The quantitative human OGT (glycosylated) sandwich ELISA kit is designed to detect human O-linked-N-acetylglucosamine transferase (OGT) levels. OGT is an enzyme that catalyzes the addition of O-GlcNAc to serine and threonine residues of target proteins, playing a crucial role in regulating various cellular processes, including signaling, transcription, and metabolism. It is involved in post-translational modifications that affect protein function. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.824 ng/mL, which can accurately detect low concentrations of OGT in the sample.

OGT

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.824 ng/mL

Quantitative sandwich ELISA

5 ng/mL-100 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

O-linked-N-acetylglucosamine transferase

OGT; O-linked-N-acetylglucosamine transferase; O-GLCNAC; O-GlcNAc transferase subunit p110

4, 10, 12, 18, 38, 52, 56, 399

The quantitative human OGT (glycosylated) sandwich ELISA kit is valuable for researchers investigating diabetes, cancer, and neurodegenerative diseases, where O-GlcNAc modifications and OGT activity are implicated. By quantifying OGT, researchers can gain insights into its role in disease pathogenesis and explore potential therapeutic targets related to O-GlcNAc signaling.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 95-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.