Quantitative Human PAR2 (Glycosylated) Sandwich ELISA Kit

The quantitative human PAR2 (glycosylated) sandwich ELISA kit is designed to detect human protease activated receptor 2 (PAR2) levels. PAR2 is a G protein-coupled receptor that is activated by certain proteases. PAR2 activation is involved in various cellular responses, which plays a role in inflammation and pain signaling. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.100 ng/mL, which can accurately detect low concentrations of PAR2 in the sample.

PAR2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.100 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Protease activated receptor 2

PAR2; Protease activated receptor 2; F2RL1; GPR11; G-protein coupled receptor 11; Thrombin receptor-like 1

30, 222

The quantitative human PAR2 (glycosylated) sandwich ELISA kit is designed to measure glycosylated PAR2 levels in samples. This measurement is valuable in research related to inflammation. The kit is applied in studies of pain signaling, which helps in understanding the role of PAR2.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.