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Quantitative Human RSPO3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX787

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative human RSPO3 (glycosylated) sandwich ELISA kit is designed to detect human r-spondin 3 (RSPO3) levels. RSPO3 is a secreted protein that modulates Wnt signaling. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of RSPO3 in the sample.
Target
RSPO3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.128 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
R-Spondin 3
Alternate Names
RSPO3; R-Spondin 3; PWTSR; THSD2; Roof plate-specific spondin-3
Uniprot No.
Q9BXY4
N-Glycosylation Site
36
Application Notes
Application
The quantitative human RSPO3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated RSPO3 in samples. This quantification is important for research on Wnt signaling and developmental biology. The kit also has potential applications in investigations of cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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