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Quantitative Human SCNN1β (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX449

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Specifications
Product Description
The quantitative human SCNN1β (glycosylated) sandwich ELISA kit is designed to detect human amiloride sensitive sodium channel subunit β (SCNN1β) levels. SCNN1β is a subunit of the epithelial sodium channel (ENaC). ENaC is located in the kidneys and other tissues. It plays a crucial role in regulating sodium reabsorption. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.127 ng/mL, which can accurately detect low concentrations of SCNN1β in the sample.
Target
SCNN1β
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.127 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Amiloride sensitive sodium channel subunit β
Alternate Names
SCNN1β; Amiloride sensitive sodium channel subunit β; ENaCb; SCNEB
Uniprot No.
N-Glycosylation Site
260
Application Notes
Application
The quantitative human SCNN1β (glycosylated) sandwich ELISA kit is used to measure glycosylated SCNN1β levels in samples. This measurement is important in research related to sodium transport. The kit is applied in studies of conditions affecting the kidneys, which helps in understanding the role of SCNN1β.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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