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Quantitative Human TLR5 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX75

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Specifications
Product Description
The quantitative human TLR5 (glycosylated) sandwich ELISA kit is designed to detect human toll like receptor 5 (TLR5) levels. TLR5 is a pattern recognition receptor that recognizes bacterial flagellin, triggering an immune response to bacterial infection. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.097 ng/mL, which can accurately detect low concentrations of TLR5 in the sample.
Target
TLR5
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.097 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Toll like receptor 5
Alternate Names
TLR5; Toll like receptor 5; CD285; SLEB1; TIL3
Uniprot No.
N-Glycosylation Site
37, 46, 245, 342, 422, 595, 598
Application Notes
Application
The quantitative human TLR5 (glycosylated) sandwich ELISA kit is crucial for research investigating innate immunity, inflammatory diseases, and host-pathogen interactions. The quantitative data obtained from this kit allows for precise analysis of TLR5 levels, contributing to a better understanding of its role in immune responses and disease pathogenesis.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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