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Quantitative Mouse PF4 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX927

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Specifications
Product Description
The quantitative mouse PF4 (glycosylated) sandwich ELISA kit is designed to detect mouse platelet factor 4 (PF4) levels. PF4 is a small cytokine that is released from activated platelets. It plays a role in blood coagulation. The kit is suitable for various biological samples such as cell culture supernates, serum, plasma. Its sensitivity is 6.648 ng/mL, which can accurately detect low concentrations of PF4 in the sample.
Target
PF4
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Cell culture supernates, serum, plasma
Sample Volume
100 μL
Sensitivity
6.648 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
20 ng/mL-900 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Mouse
Full Name
Platelet factor 4
Alternate Names
PF4; Platelet factor 4; CXCL4; Chemokine C-X-C-motif ligand 4; Oncostatin-A
Uniprot No.
O-Glycosylation Site
31
Application Notes
Application
The quantitative mouse PF4 (glycosylated) sandwich ELISA kit is used to measure PF4 levels in biological samples. The assay enables the study of PF4's functions in platelet biology, coagulation processes, and inflammation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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