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Quantitative Rat BALP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX31

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Specifications Target Information Application Notes Related Products
Specifications
Product Description
The quantitative rat BALP (glycosylated) sandwich ELISA kit is designed to detect rat bone alkaline phosphatase (BALP) protein levels. It is suitable for various biological samples such as tissue homogenates, serum, or plasma. Its sensitivity is 3.911 U/L, which can accurately detect low concentrations of BALP in the sample.
Target
BALP
Kit Components
Pre-coated 96-well ELISA plate; Lyophilized standard; Biotinylated BALP antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
3.911 U/L
Detection Principle
Quantitative sandwich ELISA
Detection Range
15.60 U/L-1000 U/L
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Rat
Full Name
Bone alkaline phosphatase
Alternate Names
Alkaline phosphatase; Alkaline phosphatase 2; tissue-nonspecific isozyme; Alkaline phosphatase liver/bone/kidney isozyme; AP-TNAP; TNSALP; Alpl; Akp2; BAP; BALP
Uniprot No.
P08289
N-Glycosylation Site
140, 230, 271, 303, 430
Application Notes
Application
The quantitative rat BALP (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of BALP, helping to understand its role in cancer.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-100%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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