This ALG10 knockout HEK293T cell line provides a powerful tool for investigating the impact of disrupted N-linked glycosylation on protein folding, stability, and intracellular transport. By eliminating the ALG10-encoded alpha-1,2-glucosyltransferase, this model allows researchers to explore the consequences of N-glycan truncation in a controlled cellular environment, ideal for dissecting the complexities of glycosylation processes and their impact on protein homeostasis.

Product Type

KO Cell Lines

Species

Human

Cell Morphology

Epithelial-like, adherent

Passage Ratio

1:2~1:4

Cell Line

HEK293T

Lineage

Embryonic kidney

Specification

Cell Viability

>90%

Sterility Test

The sterility test indicated an absence of microbial growth.

Identity Test

STR identification

Mycoplasma Test

Negative

Virus Test

Negative for HIV, HBV and HCV.

Genetic Stability Testing

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

Validation

PCR, Sanger Sequencing

Culture Medium

DMEM & FBS & Penicillin/Streptomycin

Application

Functional assay

Size

1 M cells/vial*2

Product Format

Frozen

Shipping

Dry ice

Availability Status

Made to order

Handling Notes

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

Product Disclaimer

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target Information

Target

ALG10

Full Name

ALG10 alpha-1,2-glucosyltransferase

Alternative Name

ALG10A, DIE2, FLJ14751

Location

12p11.1

Gene ID

84920

Summary

This gene encodes a membrane-associated protein that plays a critical role in N-linked glycosylation by adding the third glucose residue to the lipid-linked oligosaccharide precursor. Specifically, it transfers the terminal glucose molecule from dolichyl phosphate glucose (Dol-P-Glc) to the lipid-linked oligosaccharide Glc₂Man₉GlcNAc₂-PP-Dol. This step is essential for the proper assembly of the oligosaccharide precursor necessary for N-linked glycoprotein synthesis.

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For Research Use Only.

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