GlycoErase™ MAN2A1 Knockout Jurkat Cell Line (CAT#: GLJF-0425-JF1206)

Description	MAN2A1 knockout Jurkat cell line lacks MAN2A1 protein, mannosidase alpha class 2A member 1, involved in N-glycan maturation in Golgi. MAN2A1 knockout in Jurkat cells allows for the investigation of N-glycan maturation disruption in T-cell leukemia, potentially affecting final N-glycan structures, protein folding, trafficking, and Golgi-related glycosylation events critical to T-ALL biology.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Suspension-growing
Passage Ratio	1:3~1:5
Cell Line	Jurkat cell line
Primary Disease	T-cell acute lymphoblastic leukemia (T-ALL)
Lineage	T Lymphocyte
Lineage Subtype	T-cell acute lymphoblastic leukemia (T-ALL)

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & Penicillin/Streptomycin & RPMI
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	MAN2A1
Compounds Targeting	1-Deoxymannojirimyci (Alpha Mannosidase Inhibitor) Kifunensine (Mannosidase Inhibitor) Swainsonine (Alpha Mannosidase Inhibitor)
Full Name	Mannosidase alpha class 2A member 1
Alternative Name	MANA2, MANII, GOLIM7
Location	5q21.3
Gene ID	4124
Summary	Golgi-localized glycosyl hydrolase that catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway.