GlycoErase™ SIGLEC1 Knockout HEK293T Cell Line

GlycoErase™ SIGLEC1 knockout HEK293T cell line is a human embryonic kidney-derived HEK293T line, engineered to stably express SV40 large T antigen, in which SIGLEC1 expression has been eliminated. SIGLEC1 is a sialic acid-dependent adhesion receptor implicated in immune regulation and pathogen entry. The knockout of SIGLEC1 in the HEK293T background provides a high-efficiency system for exploring glycan-mediated interactions, viral infection pathways, and immune modulation, and supports applications in transient protein expression, viral vector production, and functional genomics research.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293T

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC1

Sialic Acid Binding Ig Like Lectin 1

CD169, Sialic acid-binding Ig-like lectin 1, SIGLEC1, sialoadhesin, Sialic Acid Binding Ig Like Lectin

20p13

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. The protein plays an important role in multiple human diseases and bacterial and viral infections has been shown to enhance SARS-CoV-2 infection.