GlycoFlux™ Human SIGLEC1 Overexpression HepG2 Cell Line (CAT#: GLJF-0925-JF842)

Description	GlycoFlux™ human SIGLEC1 overexpression HepG2 cell line is a stable cell line expressing SIGLEC1, a sialic acid-binding transmembrane adhesion molecule expressed in macrophage subsets. It mediates critical cell-cell interactions, influences innate immunity, and is associated with inflammatory conditions and infection biology. Recent findings highlight its role in SARS-CoV-2 enhancement. This model supports investigations into glycan-based immune recognition, virus-host interface, and macrophage glycoimmunology.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC1
Full Name	Sialic Acid Binding Ig Like Lectin 1
Alternative Name	CD169, Sialic acid-binding Ig-like lectin 1, SIGLEC1, sialoadhesin, Sialic Acid Binding Ig Like Lectin
Location	20p13
Gene ID	6614
Summary	This gene encodes a protein from the immunoglobulin superfamily, acting as a lectin-like adhesion molecule. The protein binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed by a subpopulation of macrophages and is involved in mediating cell-cell interactions. The protein plays a crucial role in several human diseases, bacterial and viral infections, and has been shown to enhance SARS-CoV-2 infection.