GlycoErase™ SIGLEC12 Knockout HEK293 Cell Line

GlycoErase™ SIGLEC12 knockout HEK293 cell line is a human embryonic kidney model lacking SIGLEC12 expression. SIGLEC12 belongs to the SIGLEC3-like subfamily, with ITIM and SLAM-like motifs that recruit SHP1/2 phosphatases to inhibit macrophage signaling. This knockout HEK293 line provides a tractable model to investigate inhibitory receptor biology, phosphatase-mediated pathways, and immune regulation, and supports applications in signal transduction studies, antibody engineering, and viral research.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

HEK293

Embryonic kidney

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

SIGLEC12

Sialic Acid Binding Ig Like Lectin 12

S2V, SLG, SIGLECL1, Siglec-XII, Sialic Acid Binding Ig Like Lectin

19q13.41

This gene encodes a member of the SIGLEC3-like subfamily, characterized by extracellular V-set and C2-set immunoglobulin-like domains, and cytoplasmic motifs ITIM and SLAM-like. Tyrosine phosphorylation of the protein recruits SHP1 and SHP2, functioning as an inhibitory receptor in macrophage signaling regulation. The gene is located on 19q13.4 and undergoes alternative splicing to produce multiple transcript variants.