GlycoErase™ SIGLEC8 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF908)

Description	GlycoErase™ SIGLEC8 knockout HepG2 cell line is a HepG2 cell line in which SIGLEC8 expression has been removed. SIGLEC8 contains conserved inhibitory motifs such as ITIM and SAP-binding sequences, mediating immune checkpoint signaling. The knockout HepG2 background provides a disease-relevant model for studying inhibitory immune pathways and cell-cell signaling, with applications in liver immunology, cancer research, and biomarker validation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC8
Full Name	Sialic Acid Binding Ig Like Lectin 8
Alternative Name	SAF2, SIGLEC-8, SIGLEC8L, Sialic Acid Binding Ig Like Lectin
Location	19q13.33-q13.41
Gene ID	27181
Summary	SIGLECs, including SIGLEC8, are type I transmembrane proteins with unique expression patterns, mainly in hematopoietic cells. SIGLEC8, a member of the CD33-like subgroup, is localized to the 19q13.3-q13.4 region and features two conserved cytoplasmic motifs: an immunoreceptor tyrosine-based inhibitory motif (ITIM) and a SLAM-like motif that interacts with the SLAM-associated protein (SAP).