Description

GlycoFlux™ MSCV-ALG10 Viral Particle leverages a Murine Stem Cell Virus (MSCV) backbone to drive ALG10, completing the LLO by transferring the terminal glucose (Dol-P-Glc → Glc₂Man₉GlcNAc₂-PP-Dol); the replication-defective vector stably integrates into the genome for long-term expression, performing efficiently in hematopoietic stem and lymphoid progenitor models.

Product Type

Recombinant MSCV Retrovirus

Target

ALG10

Species

Human

Promotor

MSCV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MSCV vector is a common retroviral vector system used to achieve high-efficiency, stable gene delivery into target cells, such as hematopoietic stem cells. It ensures long-term, sustained expression by stably integrating the gene into the host cell's genome.

Shipping

Dispatched on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to preserve titer and stability.

Handling Notes

Store at -80°C and avoid repeated freeze-thaw cycles to preserve viral titer. For safety, handle materials in a biological safety cabinet while wearing appropriate PPE (lab coat, gloves, and eye protection).

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Titer Assay	qPCR
Mycoplasma Test	Negative
Sterility	Sterility testing confirmed no microbial contamination.
Transduction Evaluation	Creative Biolabs offers, upon request, in vitro and in vivo transduction assays to verify lentiviral targeting and to quantify transgene expression and functional output.
Insert Identity Confirmation	All Creative Biolabs retroviral vectors undergo PCR-based verification of proviral integration. Cells are transduced across a dilution series of the retroviral preparation; after several days, genomic DNA is isolated, and a defined region of the expected insert is PCR-amplified to confirm correct identity and integration.

Full Name	ALG10 alpha-1,2-glucosyltransferase
Alternative Name	ALG10A; DIE2; FLJ14751
Location	12p11.1
Gene ID	84920
Summary	Transfers the final glucose from Dol-P-Glc to Glc₂Man₉GlcNAc₂-PP-Dol, completing the N-glycan precursor for protein glycosylation.

GlycoFlux™ MSCV-Human ALG10 Viral Particle (CAT#: GLJF-1025-JF516)

Specifications

Quality Control

Target Information

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