Specifications Quality Control Target Information Related Products

Specifications

Description

GlycoFlux™ MMLV-ALG10 Viral Particle packages ALG10, the membrane glucosyltransferase that donates the terminal glucose from Dol-P-Glc to Glc₂Man₉GlcNAc₂-PP-Dol, finalizing the N-glycan precursor for calnexin/calreticulin-mediated folding. Designed to build stably transduced mammalian cell models, it enables tight control of LLO triglucosylation, folding-cycle studies, and variant rescue in dividing cells.

Product Type

Recombinant MMLV Retrovirus

Target

ALG10

Species

Human

Promotor

MMLV 5' LTR promoter

Packaging System

Retrovirus

Packaging Cell

HEK293T

Application

This MMLV vector is a common retroviral vector system that permanently integrates a gene into the host cell's genome, allowing for long-term, sustained expression. It is a popular tool for gene manipulation in dividing cells and for generating iPS cells in biomedical research.

Shipping

Shipped on dry ice with temperature held at or below -80°C.

Storage

Store at -80°C to maintain their titer and stability.

Handling Notes

Store products at -80°C, and avoid repeated freeze-thaw cycles to maintain viral titer. For safety, always handle the products in a biological safety cabinet, using appropriate personal protective equipment like lab coats, gloves, and eye protection.

Product Disclaimer

These products are for research use only and not for diagnostic or clinical use. The user assumes full responsibility for all safety protocols and compliance with relevant regulations. While we are committed to quality, Creative Biolabs makes no guarantee as to the performance of these products in a specific application.

Quality Control

Titer Assay

qPCR

Mycoplasma Test

Negative

Sterility

Sterility testing confirmed no microbial contamination.

Transduction Evaluation

On request, Creative Biolabs conducts in vitro or in vivo transduction assays to evaluate lentiviral delivery to target cells and to quantify transgene expression and function.

Insert Identity Confirmation

Creative Biolabs verifies all retroviral vectors by a PCR-based proviral integration check. In brief, cells are transduced with serial dilutions of the retroviral preparation and, after several days, genomic DNA is extracted. A predefined segment of the expected retroviral insert is then amplified by PCR to confirm correct identity and integration.

Target Information

Full Name

ALG10 alpha-1,2-glucosyltransferase

Alternative Name

ALG10A; DIE2; FLJ14751

Location

12p11.1

Gene ID

84920

Summary

Membrane protein that transfers the terminal glucose from Dol-P-Glc to Glc₂Man₉GlcNAc₂-PP-Dol, finalizing N-glycan precursor assembly.

GlycoFlux™ MSCV-Human ALG10 Viral Particle (CAT#: GLJF-1025-JF516)
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GlycoFlux™ MMLV-Human ALG10 Viral Particle