GlycoSens™ Acetylcholine (ACH) Assay Kit (for culture medium)

The GlycoSens™ Acetylcholine (ACH) Assay Kit offers an advanced quantitative methodology for evaluating acetylcholine concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Oxidation and Antioxidant Series

ACH

Substrate Mix A; Substrate Mix B; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates, biological fluids

10 μL - 50 μL

The system measures chromatic product formation derived from target acetylcholine activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of ACH levels.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Acetylcholine

Acetylcholine; ACh; Acetylcholine Standard; Acetylcholine Reagent

Found widely across biological samples, acetylcholine (ACH) is crucial for maintaining systemic homeostasis. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Consequently, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Specifically engineered for evaluating acetylcholine (ACH) dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.

Stable Signal Output; Excellent Reproducibility; Minimized Interference

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.