GlycoSens™ Acetylcholine (ACH) Assay Kit (for tissue)

The GlycoSens™ Acetylcholine (ACH) Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target acetylcholine in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.

Oxidation and Antioxidant Series

ACH

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissue homogenates, cell lysates

10 μL - 50 μL

The assay relies on the distinct spectroscopic optical characteristics of acetylcholine. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect ACH content in the sample.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Acetylcholine

Acetylcholine; ACh; Acetylcholine Standard; Acetylcholine Reagent

Acetylcholine (ACH) is an integral element required for robust cellular function and metabolism. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Notably, therefore, profiling its activity serves as a fundamental strategy for exploring complex biological phenomena.

Ideal for investigating acetylcholine (ACH) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

High Specificity; Broad Sample Compatibility; Ready-to-Use Reagents

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.