GlycoSens™ Acetylcholine (ACH) Assay Kit

The GlycoSens™ Acetylcholine (ACH) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of acetylcholine. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Fatty Acid Series

ACH

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

5 μL - 20 μL

Target acetylcholine (ACH) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target ACH.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Acetylcholine

Acetylcholine; ACH

Functioning at the core of various biological processes, acetylcholine (ACH) is a key subject of biochemical analysis. By serving as a metabolic node, it integrates diverse biochemical inputs into unified physiological responses. Moreover, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Ideal for investigating acetylcholine (ACH) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.

Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.