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The GlycoSens™ Aldehyde Dehydrogenase (ALDH) Activity Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of aldehyde dehydrogenase (ALDH) activity levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.
Quantification of aldehyde dehydrogenase activityis established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.
Detection Time
Within 1 hour
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Aldehyde Dehydrogenase
Alternate Names
Dehydrogenase; ALDH; Aldehyde Dehydrogenase
Application Notes
Background
Aldehyde dehydrogenase (ALDH) activity is an integral element required for robust cellular function and metabolism. It is responsible for driving the oxidative conversion of its substrate, typically utilizing NAD or FAD as cofactors. As a result, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.
Application
Specifically engineered for evaluating aldehyde dehydrogenase activity dynamics in plant physiology, stress adaptation signaling, and cellular homeostasis research.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.